I have checked the 20th Anniversary laserdisc against the 25th Anniversary DVD (the Blu-ray copy I have does not have an audience participation track).

The audience participation track is the same recording as that on the DVD as it is on the laserdisc, but it's mixed differently, with the film soundtrack more prominent on the DVD, while it is barely audible on the laserdisc (which actually makes it more tedious, because you can't hear what the audience is responding to).

However, the laserdisc track starts earlier, during the Fox Video logo — on the laserdisc there is the chant to “Start the @#$%ing flick,” while on the DVD there is a spoken disclaimer during the 20th Century Fox logo that there is no audience participation during “Super Heroes.”

The 1983 “Audience Partici — pation” album, on the other hand, is a distinct recording.