Maybe. The last time I saw either of them in anything was Who Framed Roger Rabbit and Spacejam, and they were only cameos, plus both movies were parodying classical animation to a degree. But like I said: who knows. They do still endure, but they are not an immediate part of pop culture like they were from 1935-1965. Discussions like this are great fun but when you get down to it, we might as well be deciding this with roulette. I would love to get together again in 500 years and pick up this conversation. :p
On a long enough time scale, all pop culture disappears, because there is only room for so much, and what will make the cut in 500 years, 1000 years, or 10, 000 years is anyone's guess. The list gets shorter the further you go so it may help to define your timeline and parameters.