Yeah, the pitch difference is very obvious if you compare them one after another. I can immediately tell when I hear an audio sample from Star Wars that has been sped up, because I'm so used to it at the correct speed--it's most obvious in the music and in people's voices. I do not have perfect pitch, not even close--I've known people who do, and they have much more sensitive hearing than me--but I do have a good memory for sound.

I listen to a lot of Baroque music played on period instruments (Bach, Vivaldi, etc), and these ensembles often use older methods of tuning that pre-date equal temperament, and typically tune about a semitone lower than standard concert pitch, so their renditions of otherwise well known pieces come out sounding quite different than the modern ear is generally accustomed to. Going back and forth between the two can be somewhat jarring . . . but it's easy to get used to it being a certain way and then forget that it doesn't always sound like that.