Mac user here:
- You can use DVD Player to open DVD media (VIDEO_TS folders definitely, and maybe ISO files) that's stored on a hard drive. Just go to "File - Open DVD Media..." and navigate to the folder you're looking for.
- I recommend Roxio Toast for disc burning on a Mac. It'll burn both single-layer (~4.5GB) and dual-layer (~8.5GB) discs. This is what I used to burn the Revisited NTSC DVD-9. It'll burn VIDEO_TS folders and ISO files.
- I highly recommend getting Verbatim dual-layer blank discs, not any other brand. Memorex is particularly bad - only about 50% (at best) of Memorex dual-layers I try to burn are successful. Verbatims are more like 95% successful.
Hope that helps!