That depends. What was the frame rate/sample rate of your original project?
Without knowing anything, I'd say:
23.976 fps if you're working in NTSC; 25 fps if you're working in PAL
48kHz sample rate for the audio
And use a lossless codec of some sort for your master file (huffYUV as an example). It will be freaking huge, mind you, but you won't lose any quality.